Author(s): Pecsenye, K., Vadkerti, E. & Varga, Z.
Title: Temporal and spatial pattern of genetic differentiation in Isophya kraussi (Orthoptera: Tettigonoidea) in NE Hungary
Year: 2003
Volume: 49
Pages: 167-178.
Abstract: Allozyme polymorphism was studied in four samples of Isophya kraussi (Brunner von Wattenwyl, 1878), a brachipterous, sedentary tettigonoid species. Three samples were collected in the Aggtelek Karst region (Haragistya 1998, Mogyoróskuti meadows 1999 and 2001) and one in the Zemplén Mts (Gyertyán meadows 1998–1999). Enzyme polymorphism was investigated by polyacrylamide gel electrophoresis. All 10 scorable loci (Aldox, Est, Got, alpha-Gpdh, Hk, Idh, Mdh, Me, Pgi and Pgm) had alternative alleles in at least one sample. We detected a high level of polymorphism. On average, more than 60% of the investigated loci were polymorphic and the average number of alleles was about 2.1. The average heterozygote frequency was also high approximately 0.19. Large FIT values indicated a considerable level of genetic variation as well. The larger part of the total variation was explained by the within sample component and a relatively smaller portion of it was attributable to the between sample component. Many of the FIS values were positive indicating some heterozygote deficiency. In the analysis of spatial variation, we obtained highly significant FST values, which suggested strong genetic differentiation between the local populations. In contrast, the FST value calculated in the temporal analysis of variation was not significant. The average value of Nei's genetic distances between pairs of samples of geographically distinct populations was 0.071 while, that estimated within the Mogyoróskut population was 0.027. Thus, the results of all analyses indicated a higher level of differentiation among the geographically distinct populations than between the two samples collected in a single population in different years. The results of the PCA analyses fully confirmed those obtained in other analyses.
Keywords: enzyme polymorphism, genetic differentiation, Isophya kraussi, Orthoptera
Journal: Acta Zoologica Academiae Scientiarum Hungaricae
Journal abbreviation:
ISSN: 1217-8837
Publisher: Hungarian Natural History Museum, Budapest
Editor(s): Matskási, I. & Peregovits, L.
