Author(s): Németh, I.
Title: Studies on humoral antibodies in experimental infections of rabbits with Cysticercus pisiformis (Bloch, 1780). II. Localization of precipitating antibodies by immunoelectrophoresis
Title: A humorális ellenanyagok tanulmányozása nyulakban kísérletileg létesített Cysticercus pisiformis (Bloch, 1780)-fertőzöttség esetén II. A precipitáló ellenanyagok lokalizációjának vizsgálata immunelektroforézissel
Year: 1972
Volume: 5
Pages: 83-98.
Abstract: Localization of immunoprecipitins produced by rabbits in the immune response to experimental infection with Cysticercus pisiformis (Bloch, 1780), the larval form of Taenia pisiformis (Bloch, 1780) Gmelin, 1790, was studied by agar gel immuno-electrophoresis during the first 100 days of primary infection and the first 40 days of secondary infection. Rabbits, 6-7 weeks old, weighing 700-900 g were each infected orally with 500 eggs of Taenia pisiformis, and a group of them was reinfected with 10 000 eggs 60 days later. Serum samples collected at frequent intervals were tested for the presence of precipitins by agar gel double diffusion technique using “secretions and excretions” and whole saline exctracts of both Taenia pisiformis and Cysticercus pisiformis as antigens. Sera of known precipitin activity were submitted to electrophoresis in agar, and subsequently diffused against worm antigens, goat antiserum to rabbit whole serum of sheep antiserum to rabbit globulins. With all sera precipitin lines developed against the antigenic preparations at the position of IgG globulin. It appears that the precipitating antibodies produced by rabbits in response to invasion to Taenia pisiformis oncosphaeres are associated with the IgM immunoglobulin class.Journal: Parasitologia HungaricaJournal abbreviation: ISSN: 0303-688XPublisher: Népművelési Propaganda Iroda, BudapestEditor(s): Holló, F., Harsányi, L., K. Murai, É., Thoma, A. & Nemes, I.