Parasitologia hungarica

Author(s): Juhász, S.
Title: Studies on the protease inhibitor of a rumen fluke, Paramphistomum daubney
Title: A Paramphistomum daubney bendőmétely faj proteáz-inhibitorának vizsgálata
Year: 1981-1982
Volume: 14
Pages: 67-72.

Abstract: Trypsin and chymotrypsin inhibiting factor was obtained by affinity chromatography in a partially purified form from the extracts of P. daubneyi Dinnik, 1962, a fluke of the rumen of cattle. The same molecule can inhibit both trypsin and chymotrypsin by the same (or closely adjacent) active sites. The molecular weight of the inhibitor as estimated by gel chromatography is 6 000. The inhibitor is heat stable, resistant to pH changes, treatment by urea, but sensitive to 2-mercaptoethanol. The complex-formation between enzyme and inhibitor is time-dependent.

Journal: Parasitologia Hungarica
Journal abbreviation:
ISSN: 0303-688X
Publisher: Népművelési Propaganda Iroda, Budapest
Editor(s): K. Murai, É., Thoma, A. & Nemes, I.