Parasitologia hungarica

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Author(s): Majoros, G.
Title: A combined procedure of faecal examination for the parasitological testing of herbivorous farm animals
Title: Kombinált bélsárvizsgálati eljárás növényevő haszonállatok parazitiológiai vizsgálatára
Year: 1986
Volume: 19
Pages: 135-139.

Abstract: A faecal examination technique suitable for demonstrating endoparasites of herbivorous farm animals is recommended for practical use. With this technique, oocysts, helminth eggs and larvae can be demonstrated by a single microscopic examination. Soluble components and fibres are removed from the sample by aqueous decantation and filtration through a sieve, then the larvae isolated from the remainder of the faecal sample are dropped onto the egg-containing sediment. After staining with methylene blue, the combined sediment is dehydrated by centrifugation and shaken thoroughly with a high-density salt solution (a mixture of 20 parts of saturated zinc-sulphate solution and 7 parts of saturated saline; density: 1400 g/1). After repeated centrifugation, the parasites accumulate on the surface of the solution.
Keywords: demonstration of eggs and larvae, ruminant helminthology

Journal: Parasitologia Hungarica
Journal abbreviation:
ISSN: 0303-688X
Publisher: Népművelési Propaganda Iroda, Budapest
Editor(s): K. Murai, É., Thoma, A. & Nemes, I.