Parasitologia hungarica

Author(s): Upma, J. A. & Banyal, H. S.
Title: A 24,000 sediment of Plasmodium berghei induced IL-1 response in mice and exhibits protection against malaria infection
Title: A Plasmodium berghei-ből nyert 24,000 g-os üledék IL-1 választ indukál egérben és véd a maláriás fertőzéssel szemben
Year: 1998
Volume: 31
Pages: 13-21.

Abstract: Plasmodium berghei was subjected to differential centrifugation. Mice immunized with sediments (SI and SIII) emulsified in Freund’s complete adjuvant (FCA) and challenged with 1 × 10000000 live P. berghei showed that SIII imparted complete protection against infection. SIII with FCA or saponin adjuvants showed the latter to be a better adjuvant as postchallenge parasitaemia in mice remained below 2%. Immune sera obtained from the SIII-saponin group inhibited 89% merozoite invasion in vitro. High antibody level was detected by indirect immunofluorescence assay (IFA). Sera collected from S-III immunized mice showed enhanced level of IL-I. Biochemical analysis of sediments revealed SIII to be enriched in acid proteinase and acid phosphatase. The role of parasite component sedimented at 24,000 g in malaria protection is discussed.
Keywords: malaria, Plasmodium berghei, in vitro, IL-1, saponin

Journal: Parasitologia Hungarica
Journal abbreviation:
ISSN: 0303-688X
Publisher: Magyar Parazitológusok Társasága, Budapest
Editor(s): K. Murai, É., Thoma, A., Gubányi, A. & Tajti, T. Zs.